Figure 1. Binding of MVs of P. gingivalis to SBs.

Binding of P. gingivalis MVs to SBs was examined by SDS-PAGE (A: silver staining, B: WB of FimA). Five µg of the starting material from conventional purification (Crude) was incubated with same amount (20 mg, wet weight) of three SBs with different surface chemistries (SB-Epoxy, SB-COOH, and SB-NH₂). After incubating the crude MVs with SBs, unbound components were washed five times with PBS. The first and fifth unbound fractions are applied to the wells (Unbound, 1^st and Unbound, 5^th, or lanes 1 and 2). Then bound components were eluted by addition of SDS-PAGE loading buffer (Elu or lane 3). “MW” denotes the molecular weight marker. The same volume (10 µl) of samples was applied to each well of the PAGE gel. (B) Identical samples to those used in (A) were used for the WB of FimA. The location of FimA is shown by an arrow.