Table 5. AMOVA results from simulated annealing approach.

mtDNA	Source of variation	d.f.	var%	P-value
(1) Sites 3,4,5 and(2) Sites 1,2, 6–19	Among groups	1	62.9	<0.00001*
	Among populationswithin groups	16	9.84	<0.00001*
	Within populations	377	27.26	0.00196*
(1) Sites 3,4,5, (2) Sites 11–14and (3) Sites 1,2,6–10,15–19	Among groups	2	55.01	<0.00001*
	Among populationswithin groups	15	6.01	<0.00001*
	Within populations	377	38.98	<0.00001*
Microsatellites	Source of variation	d.f.	var%	P -value
(1) Sites 4,5, (2) Sites 1–3,6–11,(3) Sites 12–14 and (4) 15–19	Among groups	3	0.05	0.00391*
	Among populationswithin groups	15	0.21	0.00880*
	Within populations	1221	99.74	0.03431*

Degrees of freedom (d.f.), variance components (var), percent variation (var%) and F-statistics to test for evidence of regional genetic differentiation among Nassau grouper subpopulations using mitochondrial DNA and microsatellites. (*) denotes statistical significance of p<0.05.