Figure 3. Hemoglobin can block the Zn-PPIX internalization by Paracoccidioides.

Iron deprived Pb01 and Pb18 yeast cells were pre-incubated (+) or not (−) with hemoglobin (Hb) for 1 h. After, the cells were incubated in MMcM medium supplemented with 60 µM zinc protoporphyrin IX (Zn-PPIX) for 2 h. After this period, the cells were washed twice and observed by bright field microscopy (left panels for each strain) and by live fluorescence microscopy (right panels for each strain).