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. 2014 May 15;8(5):e2856. doi: 10.1371/journal.pntd.0002856

Figure 6. Paracoccidioides Rbt5 is a GPI-anchored protein localized in the yeast cell wall.

Figure 6

A. Cell wall fraction of Pb01 yeast cells was obtained and analyzed by Western blot using polyclonal antibodies raised against the recombinant protein Rbt5. Proteins that were obtained from the cell wall (lane 1) were extracted by HF-pyridine digestion and analyzed (lane 2). Molecular weight markers are indicated at the right side of the panel. B. Immunoelectron microscopic detection of Rbt5 in Pb01 yeast cells by post embedding methods. (1) Negative control exposed to the rabbit preimmune serum. (2 and 3) Gold particles are observed at the fungus cell wall (arrow) and in the cytoplasm (double arrowheads). Bars: 1 µm (1 and 2) and 0.5 µm (3). v: vacuoles. m: mitochondria. w: cell wall.