Table 1. Relevant Paracoccidioides Pb01 proteins that were induced in the presence of hemoglobin as detected by nanoUPLC-MSE.
| Accession numbera | Protein description | Score AVG | Peptides AVG | Fold change (Hb:Fe) | E.C. number | Subclassification | Filterb |
| METABOLISM | |||||||
| Amino acid metabolismo | |||||||
| PAAG_02163 | acetyl-/propionyl-coenzyme A carboxylase alpha chain | 249.65 | 16.33 | *** | 6.4.1.3 | Valine and isoleucine degradation | 1 |
| PAAG_07036 | methylmalonate-semialdehyde dehydrogenase | 279.14 | 14.83 | 1.60 | 1.2.1.27 | Valine, leucine and isoleucine degradation | 2 |
| PAAG_00221 | acetolactate synthase | 260.71 | 10.20 | 1.55 | 2.2.1.6 | Valine, leucine and isoleucine biosynthesis | 2 |
| PAAG_06416 | conserved hypothetical protein (alanine racemase) | 169.27 | 4.00 | *** | 5.1.1.1 | Alanine metabolism | 1 |
| PAAG_08065 | aspartate-semialdehyde dehydrogenase | 501.59 | 5.80 | 3.35 | 1.2.1.11 | Amino acid biosynthesis | 2 |
| PAAG_03138 | alanine-glyoxylate aminotransferase | 381.01 | 7.33 | 1.40 | 2.6.1.44 | Amino acid metabolism | 2 |
| PAAG_06217 | acetylornithine aminotransferase | 313.28 | 11.25 | 1.72 | 2.6.1.11 | Arginine biosynthesis | 1 |
| PAAG_06506 | aspartate aminotransferase | 253.11 | 6.50 | 1.42 | 2.6.1.1 | Aspartate and glutamate metabolism | 1 |
| PAAG_06835 | cystathionine gamma-lyase | 257.79 | 7.00 | *** | 4.4.1.1 | Cysteine biosynthesis | 1 |
| PAAG_07813 | cysteine synthase | 310.64 | 7.33 | *** | 4.2.1.22 | Cysteine biosynthesis | 2 |
| PAAG_05392 | betaine aldehyde dehydrogenase | 913.69 | 8.67 | 1.68 | 1.2.1.8 | Glycine biosynthesis | 2 |
| PAAG_01568 | glycine dehydrogenase | 193.28 | 16.00 | *** | 1.4.4.2 | Glycine degradation | 2 |
| PAAG_05406 | histidine biosynthesis trifunctional protein | 196.65 | 14.00 | *** | 3.5.4.19; 3.6.1.31; 1.1.1.23 | Histidine biosynthesis | 1 |
| PAAG_00285 | imidazole glycerol phosphate synthase hisHF | 175.08 | 19.00 | *** | 2.4.2.-; 4.1.3.- | Histidine biosynthesis | 1 |
| PAAG_09095 | ATP phosphoribosyltransferase | 1303.26 | 4.67 | 1.79 | 2.4.2.17 | Histidine biosynthesis | 2 |
| PAAG_04099 | methylcrotonoyl-CoA carboxylase subunit alpha | 179.63 | 10.00 | *** | 6.4.1.4 | Leucine degradation | 1 |
| PAAG_06387 | homoisocitrate dehydrogenase | 431.93 | 8.00 | 1.84 | 1.1.1.87 | Lysine biosynthesis | 2 |
| PAAG_02693 | saccharopine dehydrogenase | 241.77 | 12.67 | 1.43 | 1.5.1.10 | Lysine metabolism | 1 |
| PAAG_07626 | cobalamin-independent synthase | 1296.09 | 25.33 | 2.92 | 2.1.1.14 | Methionine biosynthesis | 2 |
| PAAG_06996 | G-protein comlpex beta subunit CpcB | 1162.11 | 11.33 | 2.32 | N.A. | Regulation of amino acid metabolism | 2 |
| PAAG_03613 | phosphoserine aminotransferase | 265.03 | 8.67 | 4.35 | 2.6.1.52 | Serine biosynthesis | 2 |
| PAAG_07760 | threonine synthase | 171.92 | 8.00 | *** | 4.2.3.1 | Threonine biosynthesis | 1 |
| PAAG_08668 | anthranilate synthase component 2 | 242.62 | 11.50 | *** | 4.1.3.27 | Tryptophan biosynthesis | 1 |
| PAAG_05005 | anthranilate synthase component 1 | 186.39 | 14.00 | 1.38 | 4.1.3.27 | Tryptophan biosynthesis | 2 |
| PAAG_02644 | kynurenine-oxoglutarate transaminase | 179.07 | 6.00 | *** | 2.6.1.7 | Tryptophan degradation | 2 |
| PAAG_08164 | homogentisate 1,2-dioxygenase | 323.07 | 8.25 | 1.68 | 1.13.11.5 | Tyrosine degradation | 1 |
| Nitrogen, sulfur and selenium metabolism | |||||||
| PAAG_00468 | 4-aminobutyrate aminotransferase | 1947.94 | 10.50 | 1.86 | 2.6.1.19 | Nitrogen utilization | 2 |
| PAAG_03333 | formamidase | 14545.80 | 19.33 | 1.22 | 3.5.1.49 | Nitrogen compound metabolic process | 2 |
| PAAG_05929 | sulfate adenylyltransferase | 303.58 | 9.20 | 2.39 | 2.7.7.4 | Sulfur metabolism | 2 |
| PROTEIN FATE (folding, modification, destination) | |||||||
| Protein/peptide degradation | |||||||
| PAAG_02907 | conserved hypothetical protein (ankyrin repeat protein) | 288.17 | 6.00 | *** | N.A. | Cytoplasmic and nuclear protein degradation | 1 |
| PAAG_03512 | carboxypeptidase Y | 496.47 | 6.33 | 1.23 | 3.4.16.5 | Lysosomal and vacuolar protein degradation | 2 |
| PAAG_01966 | hypothetical protein (vacuolar protease A) | 299.75 | 3.33 | 1.25 | 3.4.23.25 | Lysosomal and vacuolar protein degradation | 2 |
| CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES | |||||||
| Transported compounds | |||||||
| PAAG_01051 | conserved hypothetical protein (Pb01 Csa2) | 151.83 | 6.00 | *** | N.A. | Hemoglobin receptor | 1 |
| INTERACTION WITH THE ENVIRONMENT | |||||||
| Homeostasis | |||||||
| PAAG_05851 | cysteine desulfurase | 160.27 | 7.00 | *** | 2.8.1.7 | Iron-sulfur cluster assembly | 1 |
| PAAG_05850 | conserved hypothetical protein (cysteine desulfurase) | 395.45 | 10.25 | 1.65 | 2.8.1.7 | Iron-sulfur cluster assembly | 1 |
a
Information that was obtained from the Paracoccidioides Database (http://www.broadinstitute.org/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html).
b
filter 1 – proteins that were derived from PepFrag2; filter 2 – proteins that were derived from PepFrag1, as determined by PLGS and cited by Murad and Rech (2012).
***: proteins that were identified only in the presence of hemoglobin;
N.A.: not applicable.