Table 2. Relevant Paracoccidioides Pb01 proteins that were repressed in the presence of hemoglobin as detected by nanoUPLC-MSE.
| Accession number | Protein description | Score AVG | Peptides AVG | Fold change (Hb:Fe) | E.C. number | Subclassification | Filter |
| METABOLISM | |||||||
| Amino acid metabolism | |||||||
| PAAG_01206 | L-asparaginase | 197.88 | 5.00 | *** | 3.5.1.1 | Asparagine degradation | 1 |
| PAAG_01365 | choline dehydrogenase | 206.25 | 13.00 | *** | 1.1.99.1 | Glycine biosynthesis | 1 |
| PAAG_02935 | glycine cleavage system H protein | 404.32 | 4.33 | 0.66 | N.A. | Glycine degradation | 2 |
| PAAG_04102 | isovaleryl-CoA dehydrogenase | 330.74 | 7.00 | *** | 1.3.8.4 | Leucine degradation | 2 |
| PAAG_01974 | mitochondrial methylglutaconyl-CoA hydratase | 223.92 | 6.00 | *** | 4.2.1.18 | Leucine degradation | 1 |
| PAAG_03569 | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase | 175.60 | 6.00 | *** | 1.13.11.54 | Methionine biosynthesis | 1 |
| PAAG_08166 | 4-hydroxyphenylpyruvate dioxygenase | 203.44 | 3.00 | *** | 1.13.11.27 | Tyrosine and phenylalanine degradation | 1 |
| PAAG_00014 | dihydroxy-acid dehydratase | 312.52 | 10.83 | 0.73 | 4.2.1.9 | Valine and isoleucine biosynthesis | 2 |
| PAAG_02554 | 3-hydroxyisobutyryl-CoA hydrolase | 402.87 | 9.75 | 0.58 | 3.1.2.4 | Valine degradation | 2 |
| PAAG_01194 | 2-oxoisovalerate dehydrogenase subunit beta | 219.86 | 6.00 | *** | 1.2.4.4 | Valine, leucine and isoleucine degradation | 2 |
| PAAG_06096 | phospho-2-dehydro-3-deoxyheptonate aldolase | 1370.44 | 9.67 | 0.66 | 2.5.1.54 | Phenyalanine, tyrosine and tryptophan biosynthesis | 2 |
| PAAG_07659 | chorismate synthase | 193.84 | 3.50 | 0.61 | 4.2.3.5 | Phenyalanine, tyrosine and tryptophan biosynthesis | 2 |
| Nitrogen, sulfur and selenium metabolism | |||||||
| PAAG_04525 | glutamine synthetase | 156.10 | 9.00 | *** | 6.3.1.2 | Nitrogen metabolism | 1 |
| PAAG_07689 | NADP-specific glutamate dehydrogenase | 2006.41 | 19.83 | 0.75 | 1.4.1.4 | Nitrogen metabolism | 2 |
| Secondary metabolism | |||||||
| PAAG_00799 | uroporphyrinogen decarboxylase | 362.04 | 8.67 | *** | 4.1.1.37 | Porphyrin biosynthesis | 2 |
| PAAG_06925 | conserved hypothetical protein (glutamate-1-semialdehyde 2,1-aminomutase) | 322.93 | 7.00 | *** | 5.4.3.8 | Porphyrin biosynthesis | 2 |
a
Information that was obtained from the Paracoccidioides Database (http://www.broadinstitute.org/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html).
b
filter 1 – proteins that were derived from PepFrag2; filter 2 – proteins that were derived from PepFrag1, as determined by PLGS and cited by Murad and Rech (2012).
***: proteins that were identified only in the presence of inorganic iron;
N.A.: not applicable.