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GAS5 affects E2F1 and P21 protein levels. (A, B) Western blot analysis of E2F1, CyclinD1, P21 and cleaved caspase-3 in pCDNA3.1-GAS5 transfected SGC7901 or BGC823 cells and respective control cells. (C) Western blot analysis of E2F1, CyclinD1 and P21 after si-GAS5 transfection with MGC 803 cells. GAPDH protein was used as an internal control. * P < 0.05; ** P < 0.01.
