Table 2.
Primers used to create single nucleotide variation in nAChR α6 subunit cDNA
| Sl. no | Primer | Oligonucleotide sequence (5’ → 3’) |
|---|---|---|
| 1. |
hα6(S43P) |
ctcttccacaaactgtttCctcattacaaccagttc |
| 2. |
hα6(N46K) |
ctgttttctcattacaaGcagttcatcaggcctg |
| 3. |
hα6(D57N) |
gtggaaaacgtttccAaccctgtcacggtac |
| 4. |
hα6(R87C) |
gaaaccaatttgtggctgTgtcacatctggaatgatt |
| 5. |
hα6(D92E) |
cgtcacatctggaatgaGtataaattgcgctggg |
| 6. |
hα6(R96H) |
ctggaatgattataaattgcActgggatccaatggaatatg |
| 7. |
hα6(E101K) |
gcgctgggatccaatgAaatatgatggcattgag |
| 8. |
hα6(A112V) |
gactcttcgcgttcctgTagataagatttggaagc |
| 9. |
hα6(S156R) |
ccagctatttttaagagGtcctgccctatggatatc |
| 10. |
hα6(N171K) |
ctttttcccttttgatcatcaaaaGtgttccctaaaatttggttcctgg |
| 11. |
hα6(A184D) |
cctggacgtatgacaaagAtgaaattgatcttctaatc |
| 12. |
hα6(D199Y) |
ggatcaaaagtggatatgaatTatttttgggaaaacagtgaatg |
| 13. |
hα6(N203T) |
gatatgaatgatttttgggaaaCcagtgaatgggaaatcattgatg |
| 14. |
hα6(I226T) |
caaatacaactgttgtgaagagaCatacacagatataacctattc |
| 15. | hα6(S233C) | gatatacacagatataacctattGtttctacattagaagattgccg |
For mutants, the first amino acid (single letter code, numbering begins at the translation start methionine) designates the WT human nAChR hα6 subunit residue that is replaced with the indicated, second amino acid. In the forward primer nucleic acid sequence, capitalization indicates the nucleotide changed from the WT subunit to create the corresponding mutant.