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. 2014 May 15;9(5):e97592. doi: 10.1371/journal.pone.0097592

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© 2014 De Martin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence staining of TECs for the plasma membrane marker PKH67 (green), CRL (blue) and RAMP2 (red). When, for control purposes, the primary antibody was replaced by a non-immune PBS solution, no reactivity could be observed (not shown in the figure). (B) Distribution of fluorescence intensity (ADC units) of PKH67 (green), CLR (blue) and RAMP2 (red) in two distinct plasma membrane regions (indicated by a square).