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. 2014 May 15;9(5):e97592. doi: 10.1371/journal.pone.0097592

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© 2014 De Martin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence staining of TECs for the nuclear marker Acetyl-Histone H3 (blue) and RAMP2 (green). Cell nuclei are red stained with propidium iodide. When, for control purposes, the primary antibody was replaced by a non-immune PBS solution, no reactivity could be observed (not shown in the figure). (B) Distribution of fluorescence intensity (ADC units) of RAMP2 (green), Acetyl-Histone H3 (blue) and propidium iodide (red). The three fluorescence signals display the same nuclear distribution.