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. 2014 May 15;9(5):e97602. doi: 10.1371/journal.pone.0097602

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© 2014 Whelan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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One hundred and thirty days after being treatment with anti-CD25 antibody, mice were sacrificed and their spleens stained for CD4⁺CD25⁺ Tregs. There were no significant differences seen in the percentage of CD4⁺CD25⁺ cells between those mice that had cured of their tumour or naïve mice (n = 3 per group).