Table 1. Published examples of cell types and organisms modified by Cas9.

Cell type or organism	Cas9 form	Cell type	Reference numbers
Human cells	Cas9 nuclease	HEK293FT, HEK293T, HEK293, K562, iPSC, HUES9, HUES1, BJ- RiPS ,HeLa, Jurkat, U2OS	13-16, 47, 49-51, 54, 58, 61, 72, 83, 102, 103
	Cas9 nickase	HEK293FT, HEK293T	13, 14, 47, 49
	dCas9 (gene regulation)	HEK293FT, HEK293T	72-74, 76, 83
	dCas9 (imaging)	HEK293T, UMUC3, HeLa	84
Mouse or mouse cells	Cas9 nuclease	embryos	14, 24-26
	Cas9 nickase	embryos	47
	dCas9 (gene regulation)	NIH3T3	76
Rat	Cas9 nuclease	embryos	26, 36
Rabbit	Cas9 nuclease	embryos	27
Frog	Cas9 nuclease	embryos	28
Zebrafish	Cas9 nuclease	embryos	17, 33, 37, 62, 103
Fruit fly	Cas9 nuclease	embryos	29, 30, 63
Silkworm	Cas9 nuclease	embryos	31
Roundworm	Cas9 nuclease	adult gonads	32, 64-69
Rice	Cas9 nuclease	protoplasts, callus cells	21, 23
Wheat	Cas9 nuclease	protoplasts	21
Sorghum	Cas9 nuclease	embryos	23
Tobacco	Cas9 nuclease	protoplasts, leaf tissue	19, 20, 23
Thale cress	Cas9 nuclease	protoplasts, seedlings	19, 23
Yeast	Cas9 nuclease	S. cerevisiae	18
Bacteria	Cas9 nuclease	S. pneumonia, E. coli	8
	dCas9 (gene regulation)	E. coli	71, 72

Human Embryonic Kidney (HEK); Induced Pluripotent Stem Cells (iPSCs);