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. 2014 May 15;9(5):e97155. doi: 10.1371/journal.pone.0097155

Figure 3. Müller cells in retinal wholemounts.

Figure 3

(A) Recordings from vitreal endfeet of Müller cells. A patch pipette (MP) at the vitreal surface penetrating a single endfoot while many round- shaped endfeet with different diameters are visible. The patch clamp technique was used for recording the electrical currents and voltages as well as for fluorescent dye injection. The insert shows the differential interference contrast (DIC) image of a retinal axial section with a patch pipette positioned at the endfoot membrane. (B) Representative currents from caiman Müller cell endfeet in response to a voltage ramp of 80 ms duration from −150 mV to +150 mV (I/V-curves). The curves show the I/V relationship in different extracellular [K+], 2.5 mM and 10 mM, and the washout in 2.5 mM demonstrating classical glial sensitivity to potassium. (C) and (D) Two representative examples for Lucifer Yellow diffusion from injected endfoot (white arrowhead) to neighbor endfoot (white arrow) under control conditions. (E) Lucifer Yellow injection into a single endfoot in a retinal wholemount perfused with the gap junction blocker carbenoxolone (200 µM). The dye is not propagating to the neighboring cells.