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. 2014 May 15;9(5):e97647. doi: 10.1371/journal.pone.0097647

Figure 3. Expression of Kiss1 and GPR54 in mouse uterus during artificially induced decidualization in vivo.

Figure 3

(A) Representative uteri of oil-induced decidualization on days 5–8 of pseudopregnant. Con, uninjected oil horn served as a control; Oil, inject oil horn. (B) Quantitative PCR analysis of Kiss1 mRNA in oil-induced decidualization uteri on days 5, 6, 7, and 8 of pseudopregnancy. (C) Quantitative PCR analysis of GPR54 mRNA in oil-induced decidualization uteri on days 5, 6, 7, and 8 of pseudopregnancy. (D) Immunostaining of KISS-1 protein in oil-induced decidualization uteri on days 5, 6, 7, and 8 of pseudopregnancy. (E) Immunostaining of GPR54 protein in oil-induced decidualization uteri on days 5, 6, 7, and 8 of pseudopregnancy. Upper panel show original magnification ×100. Sections in upper panel were magnified in the bottom with higher magnification of ×400, respectively. All experiments were repeated three times. Data are shown as means ± SEM. *p<0.05, **p<0.01 compared with control. Scale bar, 50 µm. Negative control, without primary antibody; Con, control; ge, glandular epithelium; le, luminal epithelium; de, deciduas.