Table 1. Sequence of the primers used.
| Name | Sequence (5′-3′) | Locus | Putative miRNA target |
| MADS_F | AAGATAGAGAATCCDACDAACD | MADS-box | |
| OitaDEF1F | CCTTCGCAGGGAGATAAGGCAAAGGA | OitaDEF1 | |
| OitaDEF2F | CCTTCGGAAGGAGATAAGGCAGAGGA | OitaDEF2 | |
| OitaDEF3F | CCTGAGGAGGGAGATAAGGCAGAGAA | OitaDEF3 | |
| OitaDEF4F | TCTGAGGAGGGATGTAAGACAGAGGA | OitaDEF4 | |
| OitaDEF1R1 | TCATGCATAAGGGCCCTGTATACTTC | OitaDEF1 | |
| OitaDEF2R1 | TCATGCACTAGGGCCATGCACATTTC | OitaDEF2 | |
| OitaDEF3R1 | TCACGGAGTAAGCTCTTGTGGGTTTC | OitaDEF3 | |
| OitaDEF4R1 | TCACGCAGCAAATTATGGTGTGTCTC | OitaDEF4 | |
| OitaDEF1R2 | GTAAGTGTCTGTTTGCGTGGCGATCA | OitaDEF1 | |
| OitaDEF2R2 | GTAAGTGTCAGTTTGGGTAGCGATCA | OitaDEF2 | |
| OitaDEF3R2 | GTATGTATCAGTCTGGGTGCTAATGC | OitaDEF3 | |
| OitaDEF4R2 | ATAGGTGTCTGTCTGCGTACTGATTA | OitaDEF4 | |
| OitaActF | TCGCGACCTCACCAATGTAC | OitaAct | |
| OitaActR | CCGCTGTAGTTGTGAATGAATAGC | OitaAct | |
| IN_3340 | TCTCGGACCAGGCTTCATTCC | miR166 | Leucine-zipper transcription factor |
| IN_36629 | TCGCTTGGTGCAGGTCGGGA | miR168 | AGO1 |
| IN_33620 | TTCCACAGCTTTCTTGAACTG | miR396 | Growth regulating factor 4 |
| IN_16410 | TCGATAAACCTCTGCATCCGG | miR162 | Dicer1-like |
| IN_32138 | AAGCTCAGGAGGGATAGCGCC | miR390 | TAS3 |
| IN_30974 | CAGCCAAGGATGACTTGCCGA | miR169 | NF-YA |
| IN_33680 | TGCCTGGCTCCCTGTATGCCA | miR160 | Auxine response factor |
| IN_26041 | TTTTGCTCAAGACCGCGCAAC | miR5179 | DEF-like genes |
| IN_20892 | ATATGAGCTCAAATCTAAGCTTG | Unknown miRNA | Leucine-rich repeat protein kinase |
| IN_27201 | AAACTCTCTGAAATCACCCGAGAGG | Unknown miRNA | Transmembrane kinase |
| 5.8S | ACGTCCGCCTGGGCGTCAAGC | Ribosomal 5.8S |
Sequence of the primers used to isolate the DEF-like cDNAs, to analyze their tissue expression and the expression of the selected conserved and novel miRNAs of O. italica. Relative to the selected miRNAs, the putative targets are listed (best score).