Figure 8. Enzymatic characteristics of recombinant SjM2DH in cell-free bacterial extracts.
A, relative activity in fructose reduction and mannitol oxidation directions. B, time-course evaluation (0–25 min) of reductive activity on fructose. C, influence of different pH (4.5–10.5) on the activity of SjM2DH. D, influence of temperature (20–55°C) on SjM2DH activity. E, influence of metal ion on the activity of SjM2DH.