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. 2014 May 15;9(5):e97724. doi: 10.1371/journal.pone.0097724

Figure 1. Generation and characterization of transgenic mice expressing mouse PAR1 transgene under control of mouse GPIbα promoter (GPIbα-Tg-mPAR1).

Figure 1

(A) Schematic representation of the transgene construct. The cDNA for mouse PAR1 was inserted into a vector containing the mouse GPIbα, promoter small-t intron of simian virus 40 (SV40) in the 5′-untranslated region (UTR) and SV40 polyadenylation (polyA) sequence in the 3′ -UTR. (B) Representative genotyping from GPIbα-Tg-mPAR1. The control PCR reactions used primers specific for the bradykinin B2 receptor (BkB2). (C) Platelet aggregation in response to thrombin (100 nM) or SFLLRN (50 µM) expressed as a percentage of the maximal light transmission. The results are the mean of six independent experiments.