Figure 1. Identification of E2F1 target genes requiring Jab1/CSN5 for expression. Control and shJab1 REF52 cells were infected with equal multiplicities of infection (25 min.o.i.) of control or E2F1-expressing adenovirus. RNA was harvested from infected cells and analyzed by rat Affymetrix arrays (RAE230A). E2F1 target genes induced at least 2-fold were sorted based on the effect that loss of Jab1/CSN5 had on E2F1-dependent expression. FI refers to “fold-induction” by E2F1 compared with CMV infection in control REF52 cells. shJAB1 is the percent expression of the gene in shJAB1 REF52 cells compared with E2F1-induction of the gene in control cells.