PrepaCyte-CB processing leaves samples with the highest clonogenic potential both post processing and post thaw. Whole blood has on average 10.22 CFU per 108 cells (SD±3.80). Post processing PrepaCyte-CB leaves the greatest number of CFU at 13.28 per 108 cells (SD±5.36) compared to Hetastarch which leaves 11.53 per 108 cells (SD±1.65), Sepax which leaves 11.10 per 108 cells (SD±2.89), plasma depletion which leaves 8.59 per 108 cells (SD±4.74), and density gradient which leaves 5.55 per 108 cells (SD±3.36). Samples processed with PrepaCyte-CB have a significantly greater clonogenic potential than all other methods except Hetastarch (p=<0.01). Post thaw PrepaCyte-CB again leaves the greatest number of CFU at 9.23 per 108 cells (SD±2.31) compared to Hetastarch which leaves 3.94 per 10 8 cells (SD±1.06), Sepax which leaves 6.08 per 108 cells (SD±2.15), plasma depletion which leaves 5.70 per 108 cells (SD±1.44), and density gradient which leaves 1.33 per 108 cells (SD±2.33). Samples processed with PrepaCyte-CB have a significantly greater clonogenic potential than all other methods (p=<0.01).