Table 1.
Antibody cocktails used for cell separation assessment: flow cytometry
| Antibody | Type | Volume (μl) | Cat. no. | Distributor | Tube no. |
|---|---|---|---|---|---|
| CD45 (APC Cy7) | IgG1 | 5 | 557833 | BD Biosciences | 1, 2, 3, 4 |
| CD235a (PE) | IgG2b | 5 | 555570 | BD Pharmingen | 1, 2, 3, 4 |
| 7AAD | IgG1 | 5 | 559925 | BD Pharmingen | 1, 2, 3, 4 |
| CD14 (Pacific Blue) | IgG1a | 5 | 558121 | BD Biosciences | 1, 2 |
| CD33 (PerCP-Cy5.5) | IgG1 | 20 | 333146 | BD Biosciences | 1, 2 |
| CD7 (FITC) | IgG1 | 20 | 555360 | BD Pharmingen | 1, 2 |
| CD4 (Alexa 700) | IgG1 | 5 | 557922 | BD Pharmingen | 1, 2 |
| CD25 (PE-Cy 7) | IgG1 | 5 | 557741 | BD Pharmingen | 1, 2 |
| CD3 (Biotin)c | IgG2a | 20 | 555338 | BD Pharmingen | 1 |
| CD8 (Alexa 647) | IgG1 | 5 | 557708 | BD Pharmingen | 1 |
| CD19 (Biotin)c | IgG1 | 20 | 555411 | BD Pharmingen | 2 |
| CD56 (APC) | IgG1 | 20 | 555518 | BD Pharmingen | 2 |
| CD34 (PE Cy7) | IgG1 | 5 | 348811 | BD Sciences | 3 |
| CD133 (APC) | IgG2b | 5 | 130-090-854 | Miltenyi Biotec | 3 |
| CD38 (Biotin)c | IgG1 | 5 | ab30418 | AbCam | 3 |
| CD90 (FITC) | IgG1 | 5 | 555595 | BD Biosciences | 3 |
| CD117 (unconjugated)b | IgG1 | 5 | 313202 | Bio legend | 3 |
| CD41 (unconjugated)a | IgG1 | 5 | ab15021 | AbCam | 3 |
| Lineage 1 (FITC) | IgG1/2b | 20 | 340546 | BD Biosciences | 4 |
| CD123 (PerCP-Cy5) | IgG2a | 20 | 558714 | BD Pharmingen | 4 |
| CD11c (PE) | IgG1 | 5 | 555392 | BD Pharmingen | 4 |
| HLA-DR (Alexa 700) | IgG2a | 5 | 307626 | Bio legend | 4 |
| HLA-DQ (Biotin)c | IgG2a | 5 | ab24265 | AbCam | 4 |
| HLA-ABC (Pacific Blue) | IgG2a | 5 | 311418 | Bio legend | 4 |
| HLA-G (unconjugated)a | IgG1 | 5 | ab7758 | AbCam | 4 |
| CD16 (PE-Cy7) | IgG1 | 5 | 335823 | BD Biosciences | 4 |
Unconjugated antibodies were labelled using a Zenon labelling complex from Invitrogen;
b
Alexa405 z25313 or
a
Alexa430 z25301.
c
The biotinylated antibodies were labelled using Quantum dots 605, also from Invitrogen, conjugated to streptavidin.