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Representative UPLC–ESI-MS chromatograms of ofloxacin and the IS. (A) Blank aqueous humor, (B) aqueous humor spiked with ofloxacin (2 μg mL−1) and IS (0,4 μg mL−1) (C) aqueous humor sample collected after four drops administration of topical ofloxacin (SIR: 362.84 and 468.46 ofloxacin and IS respectively). Y-axis shows the % relative intensity and X-axis the elution time. The tR for ofloxacin and IS are 1.67 and 1.35 minutes, respectively.
