Figure 4. HCMV gp68 and gp34 inhibit FcγRIII activation by rituximab antibody isotypes.
CD20 transfected 293T cells were infected for 16/cell of VACV wt or rVACV expressing gp68, gp34 or MULT-1 as a control. After opsonization with 1 µg/ml of each antibody isotype for 30 min. and removing of unbound antibody by washing, cells were co-cultivated with 1×105 BW:FcγRIIIA-ζ reporter cells per well for 16 h before supernatants were collected and mIL-2 was determined by ELISA. Each value represents three replicates; means with standard deviations (error bars) are shown for 2 independent experiments. Significance of results (Student's t-test) are presented in Table S1 as *: p<0.05 **: p<0.01 ***: p<0.001.