Skip to main content
. 2014 May 15;10(5):e1004131. doi: 10.1371/journal.ppat.1004131

Figure 6. The HCMV vFcγRs gp68 and gp34 bind antigen-IgG complexes.

Figure 6

(A) Schematic representation of the ‘antibody bipolar bridging’ model. (B) Lysates of SKOV-3 cells containing the heterocomplex of vFcγR-FLAG, antibody and antigen are immunoprecipitated using an anti-FLAG agarose. SKOV-3 cells expressing HER2 antigen on the surface were infected with rVACV expressing the vFcγRs before opsonized with the trastuzumab antibody (bipolar bridging-antibody) (T) or an isotype control IgG1 antibody, palivizumab (P). Lysates were prepared after incubation of infected cells with antibody. An anti-Flag agarose IP was performed and retrieved antigens were detected in western blot with anti-ErbB2-specific mAb recognizing human HER2, anti-human IgG, and anti-Flag (M2, Sigma-Aldrich) detecting the Flag-tagged vFcγRs. Equal expression of HER2 in cell lysates was verified by western blot analysis with an anti-ErbB2-specific rabbit mAb which detects human HER2 (bottom).