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. 2014 May 15;10(5):e1004132. doi: 10.1371/journal.ppat.1004132

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© 2014 Naffin-Olivos et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) and (B) Hip1 and Hip1(S228A) proteins were purified by gravity column chromatography and anion exchange chromatography. Top and bottom panels show the anion exchange column elution peak profiles of recombinant Hip1 and Hip1(S228A) respectively. The purity of the eluted protein was checked by SDS-PAGE analysis after each purification procedure. The arrows indicate the elution fractions used in subsequent assays: B6 for Hip1 and A12 for Hip1(S228A). (C) CD spectra of Hip1 and Hip1(S228A) mutant.