Figure 1. Experimental setups (arrows indicate the direction of entering ants).

(a) Foraging area consisting of a soil deposition site (left) and a foraging arena (right). Here the arrow indicates the entry to experimental arena(s). (b) Nest sites for the temperature induced relocation experiment (Experiment 1): left – nest site with cut-out tunnel, at room temperature; right – nest site with cut out tunnels and chamber containing fungus and brood, placed on a cooling plate. (c) Setup for humidity induced relocation experiment (Experiment 2): left – humidified nest with moistened pebbles; right – open foraging arena with 4 experimental platforms. Ants were placed into the foraging arena at the beginning of the experiment. (d) Plaster nest sites for fungus relocation experiment: left – nest site with 2 small chambers, one containing brood (Experiment 3); right – nest site with 1 big chamber, one side containing brood (Experiment 4). (e) Clay nest sites for digging experiments, only one tunnel containing brood (Experiments 5 and 6).