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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: J Neurosci Methods. 2009 Jul 21;184(1):25–36. doi: 10.1016/j.jneumeth.2009.07.015

Figure 3. Induction of d4EGFP in the barrel cortex by sensory-stimulation.

Figure 3

All but two D1 and D2 facial whiskers were trimmed, as shown in the schematic drawing, and cortical d4EGFP expression was analyzed 24 hours later. Anti-GFP immunostaining visualized with FITC-conjugated secondary antibodies revealed prominent induction of d4EGFP expression in the D1 and D2 columns in the trimmed, but not in the control untrimmed, animal. The scale bar = 1 mm. Cytochrome oxidase (co) staining (left in two panels) was used to visualize individual columns in the barrel cortex, which are organized in rows A though D; position of the D1 and D2 columns are marked in the image.