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. 2014 Apr 21;14(2):100–106. doi: 10.4110/in.2014.14.2.100

Figure 1.

Figure 1

S. flexneri evade and evoke host cell death in the small intestine without inflammation. (A) Hematoxylin and eosin (H&E) staining of terminal ileum 1 and 24 h after oral infection with 5×109 CFU wild-type M90T or T3SS mutant M90TΔipaB4 (n=5 per group). (B) Periodic acid-Schiff (PAS) staining of terminal ileum 1 and 3 hours after oral infection with 5×109 CFU M90T. Arrows indicate secretory granules of crypt Paneth cells and mucin of goblet cells (n=3 per group). (C) Localization of GFP expressing M90T in the villi and crypts of the terminal ileum 1 h after oral infection (n=3 per group).