Figure 2.

Miniature Neurotransmission Is Required for Normal Synaptic Terminal Development

(A–D) Representative traces of eEPSPs (above) and mEPSPs (below and inset) from (A) control (dglurIIA^+/−,IIB^Df/−), (B) iGluR^WT (dglurIIA^Hypo/−,IIB^Df/−;+/genomic-dglurIIA^WT), (C) iGluR^MUT (dglurIIA^Hypo/−,IIB^Df/−;+/genomic-dglurIIA^E783A), and (D) iGluR^MUT+UAS-dGluR^WT (dglurIIA^Hypo/−,IIB^Df/−,G14-Gal4;UAS-dglurIIA^WT/genomic-dglurIIA^E783A).

(E and F) Quantification of (E) eEPSP integral (n ≥ 8) and (F) mEPSP integral (n ≥ 8).

(G and H) Quantification of the NMJ (G) synaptic terminal area and (H) bouton size index (n ≥ 22). All quantification data are normalized to control.

(I–L) Representative NMJ terminals and boutons (inset) labeled with Dlg (green) and HRP (red). Scale is the same in (I)–(L) and in the insets of (I)–(L).

Error bars indicate ±SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figures S3 and S4.