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. 2014 May 7;82(3):618–634. doi: 10.1016/j.neuron.2014.03.012

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

The Requirement of Miniature Neurotransmission for Synapse Development Is Independent of Evoked Neurotransmission and Requires Ionotropic Glutamate Receptor Activity

(A–F) Representative traces of eEPSPs (above) and mEPSPs (below and inset) from (A) iGluR^WT (dglurIIA^Hypo/−,IIB^Df/−;+/genomic-dglurIIA^WT), (B) iGluR^MUT (dglurIIA^Hypo/−,IIB^Df/−;+/genomic-dglurIIA^E783A), (C) iGluR^MUT + UAS-PLTXII (dglurIIA^Hypo/−,IIB^Df/−,OK319-Gal4;UAS-PLTXII/genomic-dglurIIA^E783A), (D) iGluR^MUT + UAS-δACTX (dglurIIA^Hypo/−,IIB^Df/−,OK319-Gal4;UAS-δACTX/genomic-dglurIIA^E783A), (E) iGluR^MUT + UAS-rGluK2 (dglurIIA^Hypo/−,IIB^Df/−,G14-Gal4;UAS-rGluK2/genomic-dglurIIA^E783A), and (F) iGluR^MUT + UAS-rGluK2 + UAS-PLTXII (dglurIIA^Hypo/−,IIB^Df/−,G14-Gal4,OK319-Gal4;UAS-rGluK2 /genomic-dglurIIA^E783A,UAS-PLTXII).

(G and H) Quantification of (G) eEPSP integral (n ≥ 8) and (H) mEPSP integral (n ≥ 8).

(I and J) Quantification of the NMJ (I) synaptic terminal area and (J) bouton size index (n ≥ 27). All quantification data are normalized to control (iGluR^WT).

(K–P) Representative NMJ terminals and boutons (inset) labeled with Dlg (green) and HRP (red). Scale is the same in (K)–(P) and in the insets of (K)–(P).

Error bars indicate ±SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S4.