Figure 4.

Increasing Miniature Neurotransmission Promotes Synaptic Terminal Expansion

(A–G) Representative traces of eEPSPs (above) and mEPSPs (below) from (A) control (cpx^Df/+), (B) cpx^−/− mutant (cpx^Df/−), (C) cpx^−/− + UAS-Cpx (UAS-Cpx/+;OK6-Gal4/+;cpx^Df/−), (D) cpx^1257/− mutant (cpx^Df/1257), (E) cpx^−/− + UAS-PLTXII (UAS-PLTXII/OK319-Gal4;cpx^Df/−), (F) cpx^−/− + UAS-dGluR^DN (G14-Gal4/+;cpx^Df/-,UAS-dglurIIA^E783A), and (G) cpx^−/− + Vglut-RNAi (UAS-Vglut-RNAi^KK/OK6-Gal4;cpx^Df/−).

(H and I) Quantification of (H) eEPSP integral (n ≥ 8) and (I) mEPSP integral (n ≥ 11).

(J and K) Quantification of the NMJ (J) synaptic terminal area and (K) bouton size index (n ≥ 23). All quantification data are normalized to control.

(L–R) Representative NMJ terminals and boutons (inset) labeled with Dlg (green) and HRP (red). Scale is the same in (L)–(R) and in the insets of (L)–(R).

Error bars indicate ±SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S5.