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S229L Pol β has the same overall structure and thermal stability as WT Pol β. A, circular dichroism spectra of 1 μm WT (solid line) or S229L (dashed line) Pol β in 10 mm K2PO4 at 22 °C. B, thermal denaturation of 1 μm WT (solid line) or S229L (dashed line) Pol β analyzed in 10 mm K2PO4 at 222 nm.
