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S229L has binding affinity for 1-bp gapped DNA similar to that of WT. Various concentrations of purified WT (filled circles) or S229L (open triangles) protein were incubated with 0.1 nm 45 AG DNA substrate (Table 1) for 15 min at room temperature and then resolved by native PAGE. (WT KD(DNA) = 4.1 ± 0.8 nm; S229L KD(DNA) = 4.1 ± 0.3 nm). In reference to the WT data: This research was originally published in Ref. 8.
