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. 2014 Mar 25;289(20):13708–13716. doi: 10.1074/jbc.M114.550400

TABLE 1.

DNA Substrates

The template base is in boldface.

Substrate Sequence Assay
45AG 5′-GCCTCGCAGCCGTCCAACCAAC CAACCTCGATCCAATGCCGTCC-3′ Gel mobility shift assay
3′-CGGAGCGTCGGCAGGTTGGTTGAGTTGGAGCTAGGTTACGGCAGG-5′
3C2M45 5′-GCCTCGCAGCCGGCTGATGCGC GTCGGTCGATCCAATGCCGTCC-3′ Pre-steady-state kinetics; single-turnover kinetics
3′-CGGAGCGTCGGCCGACTACGCGGCAGCCAGCTAGGTTACGGCAGG-5′
LPSD 5′-CTGCAGCTGATGCGCUGTACGGATCCCCGGGTAC-3′ BER assay
3′-GACGTCGACTACGCGGCATGCCTAGGGGCCCATG-5′