FIGURE 1.

Ikaros binds to a specific site in the first intron of PP2A. A, schematic of the gene structure of IKZF1. The green boxes represent the introns. The sequence containing the variant site is depicted in red and an arrow. B, biotin-conjugated, intron-specific oligo (sp. oligo) or a random control oligo were used in pulldown assays with Jurkat cell nuclear extracts. The eluates were run on a gel and probed with anti-Ikaros antibody. This figure is representative of four independent experiments. IB, immunoblot.