FIGURE 2.

Ikaros binding reduces PP2A promoter activity. A, schematic of the luciferase reporter construct used in the assays. Luc, luciferase. B, 2 million 293T cells were transfected with either the reporter plasmid by itself (250 ng) or in combination with different amounts of the Ikaros expression vector (50, 100, or 200 ng) using Lipofectamine 2000. Twenty-four hours after transfection, cells were collected and lysed, and luciferase activity was quantified by using the Dual-Luciferase assay system. Renilla activity was used for normalization. The results represent the mean ± S.D. of three observations. RLU, relative light unit; cntrl, control. C, similar experiments were conducted with the mutant (mut) reporter construct in which the Ikaros binding site was deleted. The results represent mean ± S.D. of three observations.