Effect of ischemia/reperfusion on LDH release in hearts from TspoloxP and Myh6Cre/Esr1;TspoloxP mice.
A, tamoxifen was administered to mice as indicated, followed by sacrifice and heart isolation 3 days after the last tamoxifen injection. B, top, Western blot analysis of heart homogenates prepared from Myh6Cre/Esr1;TspoloxP mice treated with vehicle (lanes 1 and 2) or tamoxifen (lanes 3 and 4); each lane refers to one mouse. Bottom, LDH activity released in the coronary effluent during postischemic reperfusion (percentage of total LDH). Values are mean ± S.D. (error bars) of six independent experiments.