TABLE 1.
Effect of treatment with PK11195, Ro5-4864 and PP IX on the CRC/CRC0 of TspoloxP and AlbCre;TspoloxP mouse liver mitochondria
Mitochondria (1 mg in 2 ml of assay buffer supplemented with 5 mm succinate plus 1 μm rotenone) were incubated as described under “Experimental Procedures” in the presence of the indicated concentrations of TSPO ligands. The CRC was determined using Calcium Green-5N, and data refer to the mean ± S.D. of 4–6 experiments.
| Ligand | CRC/CRC0 |
|
|---|---|---|
| TspoloxP | AlbCre;TspoloxP | |
| PK11195 (100 μm) | 0.55 ± 0.08 | 0.53 ± 0.10 |
| Ro5-4864 (100 μm) | 0.71 ± 0.05 | 0.68 ± 0.08 |
| PP IX (8 μm) | 0.68 ± 0.10 | 0.71 ± 0.08 |