TABLE 2.
Accumulation of porphyrins by TspoloxP and AlbCre;TspoloxP mouse liver mitochondria
Mitochondria (1 mg in 2 ml of assay buffer supplemented with 5 mm succinate plus 1 μm rotenone) were incubated as described under “Experimental Procedures” in the presence of the indicated amounts of porphyrins. Porphyrin accumulation was determined spectrofluorimetrically, and data refer to the mean ± S.D. of three independent experiments performed in duplicate.
| Porphyrin added | Porphyrin accumulated |
|
|---|---|---|
| TspoloxP | AlbCre;TspoloxP | |
| nmol × mg−1 | nmol × mg−1 | |
| PP IX | ||
| 1.00 | 0.72 ± 0.04 | 0.74 ± 0.05 |
| 2.00 | 1.44 ± 0.03 | 1.44 ± 0.05 |
| 3.00 | 2.15 ± 0.02 | 1.79 ± 0.26 |
| DP IX | ||
| 1.00 | 0.56 ± 0.02 | 0.51 ± 0.05 |
| 2.00 | 1.10 ± 0.12 | 1.03 ± 0.13 |
| 3.00 | 1.66 ± 0.18 | 1.53 ± 0.21 |
| HP IX | ||
| 1.00 | 0.30 ± 0.02 | 0.31 ± 0.07 |
| 2.00 | 0.60 ± 0.03 | 0.53 ± 0.01 |
| 3.00 | 0.92 ± 0.11 | 0.83 ± 0.05 |