FIGURE 2.

Activation of EA chimeras by cysteamine. a and b, representative current traces for EA_ELIC (a) and EA_α7 (b). Bars over the trace indicate the length of application and cysteamine concentrations. Horizontal and vertical scale bars indicate 1 min and 0.1 μA current, respectively. c, cysteamine response curves for the functional EA chimeras. Current is expressed as a fraction of maximal current, n ≥ 5 oocytes. Error bars indicate S.D. Data are fit to Hill equations with the following parameters: ■, EA_ELIC, EC₅₀ = 1.0 ± 0.02 mm; ●, EA_α7, EC₅₀ = 1.3 ± 0.1 mm; ▵, EA_ELIC⁵, EC₅₀ = 0.4 ± 0.02 mm; □, EA _α7¹, EC₅₀ = 2.2 ± 0.1 mm; ○, EA_α7², EC₅₀ = 2.6 ± 0.1 mm. The corresponding Hill coefficients are 1.7 ± 0.1, 1.3 ± 0.1, 1.5 ± 0.1, 1.9 ± 0.1, and 2.4 ± 0.2, respectively.