TABLE 1.
EA chimeras
The sequences for each ECD-TMD interface element modified in this study and their EC50s for cysteamine are indicated. Residues from α7nAChR are indicated in bold. Residues conserved between the two sequences are italicized. Residues within the TM2-3 linker shown in the structures of ELIC (PDB ID code 3RQW) and α7nAChR (PDB ID code 2MAW) are underlined. A dash indicates no current detected.
| Construct | Loop 2 | Loop 7 | Loop 9 | Pre-TM1 | TM2-3 linker | C terminus | Cysteamine EC50 (mm) |
|---|---|---|---|---|---|---|---|
| ELIC | NTLE | FRL | EEIDEWW | NPS | SNILPRLPYTT | RGITL | 0.4 |
| α7nAChR | DEKN | VRW | IPNGEWD | RTL | AEIMPATSDSVPLIAQY | SAPNFVEAVSKDFA | — |
| EAELIC1 | NTLE | FRL | EEIDEWW | NPS | AEIMPATSDSVPLIAQY | SAPNFVEAVSKDFA | — |
| EAELIC2 | NTLE | FRL | EEIDEWW | NPS | AEIMPATSDSVPLIAQY | RGITL | — |
| EAELIC3 | NTLE | FRL | EEIDEWW | NPS | AEIMPRLPYSVPLIAQY | RGITL | — |
| EAELIC4 | NTLE | FRL | EEIDEWW | NPS | SNILPRLPYTVPLIAQY | RGITL | — |
| EAELIC5 | NTLE | FRL | EEIDEWW | NPS | AEILPRLPYTT----------- | RGITL | 0.4 |
| EAELIC | NTLE | FRL | EEIDEWW | NPS | APRLPYTTDSVPLIAQY | RGITL | 1.0 |
| EAα71 | NTLE | VRW | IPNGEWD | RTL | AEIMPATSDSVPLIAQY | RGITL | 2.2 |
| EA α72 | DEKN | VRW | EEIDEWW | RTL | AEIMPATSDSVPLIAQY | RGITL | 2.6 |
| EA α73 | NTLE | VRW | EEIDEWW | RTL | AEIMPATSDSVPLIAQY | RGITL | — |
| EA α74 | DEKN | FRL | IPNGEWD | RTL | AEIMPATSDSVPLIAQY | RGITL | — |
| EAα7 | DEKN | VRW | IPNGEWD | RTL | AEIMPATSDSVPLIAQY | RGITL | 1.3 |