TABLE 1.
Strains used in the study
| Strains | Description | Source |
|---|---|---|
| DH5α | E. coli strain used for cloning experiments | Invitrogen |
| BL21 (DE3) codon plus | E. coli strain used for protein expression | Stratagene |
| mc2155 | Wild type M. smegmatis strain | ATCC, 700084 |
| mc2HiT-PknB | mc2155 strain transformed with integrative vector containing ATc inducible PknB at L5 site; Hygr | This study |
| mc2::pNit | mc2155 strain transformed with pNit vector | This study |
| mc2::PknB | mc2155 strain transformed with pNit PknB | This study |
| mc2ΔpknB | M. smegmatis pknB conditional gene replacement mutant | This study |
| mc2ΔpknB::pNit | mc2ΔpknB transformed with pNit-1 vector | This study |
| mc2ΔpknB::PknB | mc2ΔpknB transformed with pNit-PknB construct | This study |
| mc2ΔpknB::PknB-K40M | mc2ΔpknB transformed with pNit-PknB-K40M construct | This study |
| mc2ΔpknB::PknB-KD | mc2ΔpknB transformed with pNit-PknB-KD (aa 1–279) construct | This study |
| mc2ΔpknB::PknB-JM | mc2ΔpknB transformed with pNit-PknB-JM (aa 1–331) construct | This study |
| mc2ΔpknB::PknB-TM | mc2ΔpknB transformed with pNit-PknB-TM (aa 1–352) construct | This study |
| mc2ΔpknB::PknB-(A-TM) | mc2ΔpknB transformed with pNit-PknB- (A-TM) construct | This study |
| mc2ΔpknB::PknB-(H-TM) | mc2ΔpknB transformed with pNit-PknB- (H-TM) construct | This study |
| mc2ΔpknB::PknB-ΔTM | mc2ΔpknB transformed with pNit-PknB-ΔTM construct | This study |
| mc2ΔpknB::PknB-PASTA1 | mc2ΔpknB transformed with pNit-PknB-PASTA1 (aa 1–422) construct | This study |
| mc2ΔpknB::PknB-PASTA2 | mc2ΔpknB transformed with pNit-PknB-PASTA2 (aa 1–490) construct | This study |
| mc2ΔpknB::PknB-PASTA3 | mc2ΔpknB strain transformed with pNit-PknB-PASTA3 (aa 1–557) construct | This study |
| mc2ΔpknB::PknA-B | mc2ΔpknB transformed with pNit PknA-B (aa 1–338 of PknA fused with aa 332–626 of PknB) construct | This study |
| mc2ΔpknB::PknH-B | mc2ΔpknB transformed with pNit PknH-B (aa 1–402 of PknH fused with aa 332–626 of PknB) construct | This study |
| H37Rv | Wild type M. tuberculosis strain | ATCC |
| H37Rv::pNit | H37Rv strain transformed with pNit-1 vector | This study |
| H37Rv::PknB | H37Rv strain transformed with pNit-PknB construct | This study |
| Rv-pptr-B | M. tuberculosis H37Rv pknB conditional expression strain under regulation of pptr promoter | Ref. 19 |
| Rv-pptr-B::pNit | Rv-pptr-B transformed with pNit-1 vector | This study |
| Rv-pptr-B::PknB | Rv-pptr-B transformed with pNit PknB construct | This study |
| Rv-pptr-B::PknB-K40M | Rv-pptr-B transformed with pNit-PknB-K40M construct | This study |
| Rv-pptr-B::PknB-KD | Rv-pptr-B transformed with pNit-PknB-KD construct | This study |
| Rv-pptr-B::PknB-JM | Rv-pptr-B transformed with pNit-PknB-JM construct | This study |
| Rv-pptr-B::PknB-TM | Rv-pptr-B transformed with pNit-PknB-TM construct | This study |
| Rv-pptr-B::PknB-(A-TM) | Rv-pptr-B transformed with pNit PknB- (A-TM) construct | This study |
| Rv-pptr-B::PknB-(H-TM) | Rv-pptr-B transformed with pNit PknB- (H-TM) construct | This study |
| Rv-pptr-B::PknB-ΔTM | Rv-pptr-B transformed with pNit PknB-ΔTM construct | This study |
| Rv-pptr-B::PknB-PASTA1 | Rv-pptr-B transformed with pNit PknB-PASTA1construct | This study |
| Rv-pptr-B::PknB-PASTA2 | Rv-pptr-B transformed with pNit PknB-PASTA2 construct | This study |
| Rv-pptr-B::PknB-PASTA3 | Rv-pptr-B transformed with pNit PknB-PASTA3 construct | This study |
| Rv-pptr-B::PknA-B | Rv-pptr-B transformed with pNit PknA-B construct | This study |
| Rv-pptr-B::PknH-B | Rv-pptr-B transformed with pNit PknH-B construct | This study |