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. 2014 Mar 18;289(20):13974–13985. doi: 10.1074/jbc.M113.543728

FIGURE 4.

FIGURE 4.

EMP2 expression promotes activation of FAK and Src. A, panels of U373 or U87MG cells were created to overexpress EMP2 or down-regulate its expression through the use of a ribozymes (RIBO) or shRNA vector. Cells were plated, harvested, and probed for activated FAK (Tyr-576/577) and activated Src (Tyr-416). *, p < 0.05, comparison by Student's t test. B, vector control LN229, GM97, and U87 or cells which overexpress EMP2 were plated for 24 h, harvested, and then probed for activated FAK and Src, total FAK, and β-actin. Left, representative Western blots. Right, semi-quantitative analysis of activated FAK and Src levels from three independent experiments; comparison by Student's t test, *, p < 0.05. C, U118, U373, and GM5 cells were transfected with an EMP2 siRNA or control siRNA, and cells were incubated for 48 h, then harvested and probed as above. Left, representative Western blots. Right, semi-quantitative analysis of pFAK and pSrc after correction for β-actin levels from three independent experiments.