TABLE 3.
Name | DNA Sequencea | kss |
---|---|---|
s−1 | ||
C/G-THF/C | FAM-5′-CATGGGCGGCATGAACCFGAGGCCCATCCTCACC-3′ | 0.49 ± 0.077 |
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′ | ||
T/G-THF/C | FAM-5′-CATGGGCGGCATGAACTFGAGGCCCATCCTCACC-3′ | 0.17 ± 0.068 |
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′ | ||
THF/G-G/C | FAM-5′-CATGGGCGGCATGAACFGGAGGCCCATCCTCACC-3′ | 0.48 ± 0.13 |
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′ | ||
THF/G-oxG/C | FAM-5′-CATGGGCGGCATGAACFXGAGGCCCATCCTCACC-3′ | 0.89 ± 0.38 |
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′ |
a F + tetrahydrofuran; underlined bases are a mispair (G:T); X + 8-oxoG. FAM indicates the 5′-fluorescence tag. The values represent the means and S.D. of at least two independent experiments.