Skip to main content
. 2014 Apr 2;289(20):13996–14008. doi: 10.1074/jbc.M114.557769

TABLE 3.

Observed steady-state rate for APE1 incision

Name DNA Sequencea kss
s1
C/G-THF/C FAM-5′-CATGGGCGGCATGAACCFGAGGCCCATCCTCACC-3′ 0.49 ± 0.077
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′
T/G-THF/C FAM-5′-CATGGGCGGCATGAACTFGAGGCCCATCCTCACC-3′ 0.17 ± 0.068
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′
THF/G-G/C FAM-5′-CATGGGCGGCATGAACFGGAGGCCCATCCTCACC-3′ 0.48 ± 0.13
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′
THF/G-oxG/C FAM-5′-CATGGGCGGCATGAACFXGAGGCCCATCCTCACC-3′ 0.89 ± 0.38
3′-GTACCCGCCGTACTTGGCCTCCGGGTAGGAGTGG-5′

a F + tetrahydrofuran; underlined bases are a mispair (G:T); X + 8-oxoG. FAM indicates the 5′-fluorescence tag. The values represent the means and S.D. of at least two independent experiments.