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Nef.GFP expression constructs containing different SH4 domains for heterologous targeting of Nef. A, schematic representation of Nef.GFP constructs used in this project. To achieve heterologous targeting, the Nef SH4 domain was replaced by the shown SH4 domains. B, Western blot analysis of Jurkat TAg T lymphocytes transfected with the Nef.GFP expression constructs. Transferrin receptor served as loading control.
