FIGURE 1.

PTEN is important for regulating mitotic timing. A, HeLa cells transfected with PTEN siRNAs (siPTEN) or luciferase siRNA (siLuc) for 1 day were treated with nocodazole for 14 h. Mitotic cells collected by shake-off were released into the cell cycle. At various times after release, cells were collected, and equal amounts of cell lysates, along with lysates from asynchronized cells (As), were prepared and blotted for PTEN, cyclin B, p-H3, and α-tubulin. Experiments were repeated at least three times, and representative data are shown. B, cyclin B and p-H3 signals in cells transfected with siPTEN or siLuc, as shown in A, were quantified, and the summarized data were plotted.