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. 2014 Apr 1;289(20):14252–14262. doi: 10.1074/jbc.M114.556308

FIGURE 3.

FIGURE 3.

Co-expression of HA-UNC-112 D382V and myc-PAT-4 P257L restores ability of UNC-112 D382V to localize to integrin adhesion sites. A, Western blot of lysates from transgenic worms expressing from a heat shock promoter HA-tagged UNC-112 D382V and either myc-tagged PAT-4 wild-type (WT) or PAT-4 P257L. Lysates were prepared after heat shock (+) or in the absence of heat shock (−) and reacted with anti-HA or anti-myc antibodies. Expression of the HA- and myc-tagged proteins depends on heat shock. B, localization of heat shock-expressed HA-tagged UNC-112 D382V in the presence of co-expressed myc-tagged PAT-4 wild type, or myc-tagged PAT-4 P257L. Adult worms were immunostained with anti-HA to detect transgenic UNC-112 and with anti-UNC-95 to visualize the optical plane of body wall muscle cells that contain dense bodies and M-lines (integrin adhesion sites). HA-UNC-112 D382V fails to localize to these structures in the presence of myc-PAT-4 WT but does localize to these structures in the presence of myc-PAT-4 P257L. Yellow arrowheads mark dense bodies, whereas yellow arrows mark M-lines.