FIGURE 2.

The polarized EC activation by Cys-mutated mCRP does not depend on EC type or culture conditions. A, the filter types used in the experiments. B–F, Cys-mutated mCRP induced polarized responses in HCAEC monolayers on collagen-coated CM filters (n = 6–8) (B); human umbilical vein endothelial cell (HUVEC) monolayers on collagen-coated PET filters (n = 3) (C); and HAEC monolayers on fibronectin-coated HA filters (n = 3) (D), collagen-coated CM filter (n = 4) (E), and collagen-coated PET filters (n = 3–4) (F). Following incubation for 4 h with 20 μg/ml Cys-mutated mCRP added apically or basolaterally, conditioned culture media from both chambers were collected, and MCP-1 and IL-8 levels were determined with specific ELISAs. EC monolayers without mCRP treatment served as controls. *, p < 0.05; **, p < 0.01.