FIGURE 3.
Bloc1s5 muted affects the expression of the BLOC-1 complex polypeptide pallidin. A, hippocampal sections from wild type, Bloc1s5+/mu, and Bloc1s5mu/mu mice were stained with antibodies against the BLOC-1 subunit pallidin and the synaptic vesicle marker VAMP2. The right panels present look-up tables (LUT) to highlight differences in pallidin expression among genotypes. B, a quantitation of pallidin immunoreactivity expressed as ratios between pallidin and VAMP2. Each dot represents an independent staining performed in four wild type and Bloc1s5mu/mu hippocampi or two Bloc1s5+/mu hippocampi. C and D, immunoblot determinations of pallidin and their quantitation in mouse hippocampi (n = 3 animals and 3 independent determinations; one-way analyses of variance followed by Dunnett's multiple comparison was used in B and D). IB, immunoblot.