FIGURE 7.

Bloc1s8^sdy/sdy and Bloc1s5^mu/mu differentially affect the expression of GABAergic interneuron markers. A and B, transcripts encoding markers of GABAergic interneurons from adult wild type, Bloc1s8^sdy/sdy (A) and Bloc1s5^mu/mu (B) hippocampi were analyzed by quantitative RT-PCR. The box plot depicts relative mRNA content with red boxes indicating double copy loss alleles. Wild type is depicted in blue in all panels. Transcripts encoding the synaptic vesicle protein synaptophysin (Sphysin) were used as loading controls. Transcripts are listed to the left of graphs in italics (n = 3 animals with determinations in at least duplicate; one-way analysis of variance followed by Dunnett's multiple comparisons). C–E, immunoblot (IB) determinations of VGAT in wild type and mutant mouse hippocampi and their quantitation normalized to actin. F, VAMP7 and VAMP2 transcript determinations in wild type and Bloc1s5^mu/mu hippocampi. Bloc1s5^mu/+ transcript levels are not shown (n = 5 animals; one-way analysis of variance followed by Dunnett's multiple comparisons).