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Purification of antimicrobial activity in silkworm hemolymph induced by the injection of heat-killed Sakai cells
The minimum inhibitory concentration of the fraction III against Sakai was 0.47 μg/ml.
| Fraction | Protein | Activity | Specific activity | Purification | Yield |
|---|---|---|---|---|---|
| mg | units | units/mg | -fold | % | |
| I. Hemolymph plasma | 450 | 10,800 | 24 | 1 | 100 |
| II. CM-TOYOPEARL | 13 | 280,000 | 21,000 | 870 | 2600 |
| III. Superose 12 | 0.58 | 128,000 | 221,000 | 9230 | 1100 |
